Retro-mode imaging and fundus autofluorescence with scanning laser ophthalmoscope of retinal dystrophies
© Parodi et al.; licensee BioMed Central Ltd. 2012
Received: 16 March 2011
Accepted: 19 March 2012
Published: 15 May 2012
Retinal dystrophies display a considerably wide range of phenotypic variability, which can make diagnosis and clinical staging difficult. The aim of the study is to analyze the contribution of retro-mode imaging (RMI) and fundus autofluorescence (FAF) to the characterization of retinal dystrophies.
Eighteen consecutive patients affected by retinal dystrophies underwent a complete ophthalmological examination, including best corrected visual acuity with ETDRS charts, blue-light fundus autofluorescence, (BL-FAF), near-infrared fundus autofluorescence (NIR-FAF), and RMI. The primary outcome was the identification of abnormal patterns on RMI. The secondary outcome was the correlation with the findings on BL-FAF and NIR-FAF.
Overall, the main feature of RMI is represented by a pseudo-3D pattern of all the lesions at the posterior pole. More specifically, any accumulation of material within the retina appears as an area of elevation of different shape and size, displaying irregular and darker borders. No precise correlations between RMI, BL-AF, and NIR-AF imaging was found.
RMI and FAF appear to be useful tools for characterizing retinal dystrophies. Non-invasive diagnostic tools may yield additional information on the clinical setting and the monitoring of the patients.
A peculiar trait of retinal dystrophies is their considerable range of phenotypic variability, which sometimes makes diagnosis and clinical staging difficult. Many attempts have been made over the past decade to characterize each dystrophy by more precise diagnostic techniques, including fundus autofluorescence (FAF) and optical coherence tomography (OCT) [1–3]. As a result, the study of clinical features and morpho-functional correlations of the retinal dystrophies has greatly improved. Nevertheless, a complete knowledge of all the clinical manifestations is yet to be achieved. Retro-mode imaging (RMI) has recently been shown to offer the best method of outlining the distinctive clinical features of several chorio-retinal disorders [4–8]. RMI is performed by a scanning laser ophthalmoscope (SLO) working at infrared wavelength to produce a pseudo-3D image. The aim of the present study is to describe patterns typical of retinal dystrophies on RMI, and to compare them with the findings obtained on FAF.
All the consecutive patients affected by retinal dystrophies referred to our department of Ophthalmology for a clinical evaluation from May 2010 to September 2010 were prospectively considered for the study. This research was approved by the institutional review board and adhered to the tenets of the Declaration of Helsinki. Each patient provided signed informed consent to the study.
The inclusion criterion was the diagnosis of retinal dystrophy. Exclusion criteria were the presence of media opacities and the identification of any other ocular or systemic disease able to give rise to chorioretinal alterations.
Each patient underwent a complete ophthalmic examination, including measurement of visual acuity, FAF and RMI. Best-corrected visual acuity (BCVA) was determined with standard Early Treatment Diabetic Retinopathy Study charts as the total number of correct letters identified at 4 m plus 30.
RMI was obtained using a F10 SLO (Nidek, Padova, Italy) [7–9]. In brief, the F10 can obtain images of the deeper retinal layers employing a 790 nm infrared laser; it is equipped with a retro-mode aperture consisting of a central stop associated with a laterally oriented oval-shaped opening, which prevents the direct light reflection crossing and allows the scattered light from only one direction to pass through the lateral aperture. The detector acquires images from surface to deep retinal layers through the lateral aperture and provides a final image appearing as a shadow of the silhouetted retino-choroidal details. This imaging technique produces pseudo-3D features offering a new means of detecting abnormalities in the deeper retinal layers and choroid.
Blue-light fundus autofluorescence (BL-FAF) was recorded using a confocal scanning laser ophthalmoscope (Heidelberg Retina Angiograph, HRA, Heidelberg Engineering, Heidelberg, Germany) using a 30° field of view and 512 × 512-pixel resolution. The optical and technical performance of the confocal scanning laser ophthalmoscope have been previously reported . In brief, the instrument uses blue laser light at 488 nm for excitation and a barrier filter at 500 nm to limit the captured light to autofluorescent structures. Near-infrared fundus autofluorescence (NIR-FAF) was obtained using the standard HRA angiograph system, using an excitation power and wavelength of 789 nm and a detection filter of >800 nm normally employed in indocyanine green angiography [3, 10, 11]. Five to nine images were taken and averaged to obtain a single image for BL-AF and NIR-AF.
The primary outcome was the identification of abnormal patterns on RMI. The secondary outcome was the correlation with the findings on BL-FAF and NIR-FAF.
The study adhered to the tenets of the Declaration of Helsinki for research involving human subjects and was approved by Ethics Committee of the Vita-Salute University, San Raffaele Scientific Institute.
A total of 18 patients with retinal dystrophies participated in the study. We included 2 patients with Best Vitelliform Macular Dystrophy (BVMD), 5 patients with Autosomal Recessive Stargardt Disease-Fundus Flavimaculatus (STGD1), 8 patients with Pattern Dystrophy (PD) of the Retinal Pigment Epithelium, 2 patients with Choroideremia (CHM), and 1 patient with Benign Concentric Annular Macular Dystrophy (BCAMD). All the diagnoses of the retinal dystrophies were confirmed by molecular genetic analysis.
Autosomal recessive Stargardt disease-fundus flavimaculatus
The 5 patients affected by STGD1 included 4 females and 1 male, with ages ranging from 22 to 67 years, and BCVA from 20/50 to 20/400. The diagnosis of STGD1 was confirmed by the identification of 2 compound heterozygous mutations in the ABCA4 gene.
Best vitelliform macular dystrophy
Pattern dystrophy of the retinal pigment epithelium
Benign concentric annular macular dystrophy
The single 57-year-old patient affected by BCAMD had a BCVA of 20/400 in both eyes. Linkage analysis showed linkage on chromosome 6p12.3-q16. RMI revealed a precise visualization of the choroidal vessels, the central area being spared. BL-AF showed an annular hypo-autofluorescence with relatively spared central retina. NIR-AF disclosed a hyper-autofluorescence corresponding to the spared retina.
Macular dystrophies are a heterogeneous group of hereditary disorders involving the macular area at different levels and with variable severity. Diagnostic tools, including fluorescein angiography, FAF, electrofunctional tests, and OCT can provide information regarding the morphological changes characterizing each dystrophy. Infrared examination of the human ocular fundus has provided interesting results in the past [12, 13]. More recently, the F10 SLO has been used non-invasively to examine the posterior pole with a retro-mode technique at an infrared wavelength, providing accurate characterization of the retinochoroidal structures [4–8]. The F10 is based on a newly developed RMI procedure using an infrared laser to provide a pseudo three-dimensional image.
The pseudo-3D image is obtained by assembling multiple pictures generated by the retinal layers at different depths and collected by the F10-detector through the retro-mode aperture. This consists of a central stop blocking the direct light reflection and a laterally oriented oval-shaped opening, which allows the scattered light spreading in only one direction to cross.
In previous reports, RMI was able to characterize the cystoid macular edemas secondary to polypoidal choroidal vasculopathy, retinitis pigmentosa or retinal vascular disorders, including diabetic maculopathy and retinal vein occlusion [4, 5]. Tanaka et al have described a specific ‘fingerprint’ pattern related to macular retinoschisis in myopic eyes, which consists of radiating retinal striae centered on the fovea and many light dots and lines that run in parallel to the striae or form a whorled pattern surrounding the radiating striae . In eyes affected by dry age-related macular degeneration, RMI featured the progressive enlargement and confluence of drusen over a short term observation, suggesting a possible role in the monitoring of subtle drusen changes in the progression of AMD .
Our results reveal that RMI is also able to detect abnormalities in retinal dystrophies. In particular, the main finding of RMI is a pseudo-3D pattern of all the lesions at the posterior pole. Any accumulation of material within the retina appeared as an elevated area, with different shapes and sizes, showing irregular and darker borders. On the other hand, atrophic regions turned out to be accurately outlined by the precise visualization of the choroidal vasculature, both in the macula and outside the macular region when the fovea was spared.
Comparison with other imaging techniques can be interesting. Indeed, BL-FAF is considered to be related to the amount of lipofuscin within the RPE cells , whereas NIR-AF patterns have been interpreted as corresponding to the extent of melanin deposition [3, 10, 11]. Interestingly, we did not find a clear correlation between RMI, and BL-FAF or NIR-FAF imaging. Bearing in mind that both BL-FAF and NIR-FAF signals correspond to the fluorescence of specific molecules (lipofuscin and melanin, respectively), the presence of other molecules contributing to the whole mass of each specific alteration cannot be visualized by the two FAF techniques. RMI thus seems to be a technique able to depict the full extension of the abnormal material amassed. Moreover, RMI provides a more precise imaging of the elevated lesions than biomicroscopic examination, precisely detecting even small alterations that can be missed in a simple ophthalmoscopic examination.
OCT can also produce 3D imaging of retinal abnormalities, providing insights useful in the diagnosis of macular diseases. In view of the fact that some alterations are located at a different depth of the retina, certain authors have argued that it is not always easy to see all the changes in a single OCT section [5, 14]. OCT and RMI may thus be considered complementary techniques, together providing a fast and non-invasive examination of the fundus.
RMI appears to be a useful tool for the characterization of retinal dystrophies. RMI may yield additional information in the diagnostic setting of macular lesions and may represent a practical diagnostic tool for cross-sectional or family studies and for the progressive monitoring of retinal dystrophy.
- Boon CJ, Jeroen Klevering B, Keunen JE, Hoyng CB, Theelen T: Fundus autofluorescence imaging of retinal dystrophies. Vision Res. 2008, 48: 2569-77. 10.1016/j.visres.2008.01.010.View ArticlePubMedGoogle Scholar
- Wirtitsch MG, Ergun E, Hermann B, et al: Ultrahigh resolution optical coherence tomography in macular dystrophy. Am J Ophthalmol. 2005, 140: 976-983. 10.1016/j.ajo.2005.06.029.View ArticlePubMedGoogle Scholar
- Parodi MB, Iacono P, Pedio M, et al: Autofluorescence in adult-onset foveomacular vitelliform dystrophy. Retina. 2008, 28: 801-7. 10.1097/IAE.0b013e31816f859d.View ArticlePubMedGoogle Scholar
- Yamamoto M, Tsujikawa A, Mizukami S, Miyoshi N, Yoshimura N: Cystoid macular edema in polypoidal choroidal vasculopathy viewed by a scanning laser ophthalmoscope: CME in PCV viewed by SLO. Int Ophthalmol. 2009, 29: 503-506. 10.1007/s10792-008-9274-7.View ArticlePubMedGoogle Scholar
- Yamamoto M, Mizukami S, Tsujikawa A, Miyoshi N, Yoshimura N: Visualization of cystoid macular oedema using a scanning laser ophthalmoscope in the retro-mode. Clin Experiment Ophthalmol. 2010, 38: 27-36. 10.1111/j.1442-9071.2010.02193.x.View ArticlePubMedGoogle Scholar
- Tanaka Y, Shimada N, Ohno-Matsui K, et al: Retromode retinal imaging of macular retinoschisis in highly myopic eyes. Am J Ophthalmol. 2010, 149: 635-640. 10.1016/j.ajo.2009.10.024.View ArticlePubMedGoogle Scholar
- Ohkoshi K, Tsuiki E, Kitaoka T, Yamaguchi T: Visualization of subthreshold micropulse diode laser photocoagulation by scanning laser ophthalmoscopy in the retro mode. Am J Ophthalmol. 2010, 150: 856-62. 10.1016/j.ajo.2010.06.022.View ArticlePubMedGoogle Scholar
- Acton JH, Cibbidge RP, King H, et al: Drusen detection in retro-mode imaging by a scanning laser ophthalmoscope. Acta Ophthalmol. 2011, 89: 404-411. 10.1111/j.1755-3768.2011.02123.x.View ArticleGoogle Scholar
- Jorzik JJ, Bindewald A, Dithmar S, Holtz FG: Digital simultaneous fluorescein and indocyanine green angiography, autofluorescence, and red-free imaging with a solid-state laser-based confocal scanning laser ophthalmoscope. Retina. 2005, 25: 405-416. 10.1097/00006982-200506000-00003.View ArticlePubMedGoogle Scholar
- Keilhauer CN, Delori FC: Near-infrared autofluorescence imaging of the fundus: Visualization of ocular melanin. Invest Ophthalmol Vis Sci. 2006, 47: 3556-3564. 10.1167/iovs.06-0122.View ArticlePubMedGoogle Scholar
- Weinberger AW, Lappas A, Kirchkamp T, et al: Fundus near infrared fluorescence correlates with fundus near infrared reflectance. Invest Ophthalmol Vis Sci. 2006, 47: 3098-3108. 10.1167/iovs.05-1104.View ArticlePubMedGoogle Scholar
- Hartnett ME, Elsner AE: Characteristics of exudative age-related macular degeneration determined in vivo with confocal and indirect infrared imaging. Ophthalmology. 1996, 103: 58-71.View ArticlePubMedGoogle Scholar
- Elsner AE, Burns SA, Weiter JJ, Delori FC: Infrared imaging of sub-retinal structures in the human ocular fundus. Vision Res. 1996, 36: 191-205. 10.1016/0042-6989(95)00100-E.View ArticlePubMedGoogle Scholar
- Yamaike N, Tsujikawa A, Ota M, et al: Three-dimensional imaging of cystoid macular edema in retinal vein occlusion. Ophthalmology. 2008, 115: 355-62. 10.1016/j.ophtha.2007.04.052.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2415/12/8/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.