A. Corneal tissue section showing absence of HSV-1 antigen in the epithelium (Negative control, Immunohistochemistry, X 500). B. Corneal tissue section showing presence of HSV-1 antigen in the epithelium (Immunohistochemistry, X 500). C. Corneal tissue section showing a multinucleated giant cell (arrow) (PAS, X 500). D. Agarose gel electrophoresis stained with ethidium bromide showing PCR amplified products using primers for a 179 bp sequence of the DNA polymerase gene specific for HSV-1/2. Lane 1: Negative control (NC); Lane 2: Patient's corneal tissue (VI 65/99); Lane 3: Positive control (PC); Lane 4: Molecular weight markers (MW).