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Figure 2 | BMC Ophthalmology

Figure 2

From: Atypical Herpes simplex keratitis (HSK) presenting as a perforated corneal ulcer with a large infiltrate in a contact lens wearer: multinucleated giant cells in the Giemsa smear offered a clue to the diagnosis

Figure 2

A. Corneal tissue section showing absence of HSV-1 antigen in the epithelium (Negative control, Immunohistochemistry, X 500). B. Corneal tissue section showing presence of HSV-1 antigen in the epithelium (Immunohistochemistry, X 500). C. Corneal tissue section showing a multinucleated giant cell (arrow) (PAS, X 500). D. Agarose gel electrophoresis stained with ethidium bromide showing PCR amplified products using primers for a 179 bp sequence of the DNA polymerase gene specific for HSV-1/2. Lane 1: Negative control (NC); Lane 2: Patient's corneal tissue (VI 65/99); Lane 3: Positive control (PC); Lane 4: Molecular weight markers (MW).

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BMC Ophthalmology

ISSN: 1471-2415

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