Figure 4From: Collection of corneal impression cytology directly on a sterile glass slide for the detection of viral antigen: An inexpensive and simple technique for the diagnosis of HSV epithelial keratitis – A pilot study Detection of HSV-1 antigen. A smear made from corneal scraping obtained from a patient with HSK showing the presence of corneal epithelial cells positive for viral antigen (cells stained brown). Many antigen negative cells are also seen (cells stained bluish purple). Note the presence of troublesome background staining. Indirect immunoperoxidase assay, × 500.Back to article page