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Figure 5 | BMC Ophthalmology

Figure 5

From: Expression of connexin genes in the human retina

Figure 5

Immunofluorescence study of Cx36 and Cx45 expression in the human retina. A) An array of red Cx36-immunosignals was detected in the inner plexiform layer (IPL) extending to the ganglion cell layer (GCL) and also in the outer plexiform layer (OPL). B) Retinal cryosections after application of only the secondary Cy3-antibodies served as control for the specificity of the Cx36 antibodies used. C) The Cx45 immunostaining (green) was very faint and the punctate pattern was prominently seen in the outer plexiform layer (OPL) but less obvious in the IPL and GCL. D) Retinal cryosections after application of only the secondary Alexa488-antibodies served as control for the specificity of the Cx36-antibodies used. All nuclei were stained blue with DAPI. Unusual large staining signals, exemplarily marked by asterisks, were considered as unspecific staining (see Methods). Scale bar: 20 μm.

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