4G8 immunoreactivity and Aβ levels were increased in cholesterol-fed rabbit retina. A. Immunohistochemical analysis of retinal sections with 4G8 (green), an antibody that detects Aβ, showed increased immunoreactivity in the cholesterol-fed rabbits compared to control. DAPI (blue) is a nuclear counter stain. Bar = 20 μm. OS, ONL, INL, GCL are outer segments, outer nuclear layer, inner nuclear layer and ganglion cell layers respectively. B, C. Aβ quantification by ELISA showed an increase in levels of Aβ40 and 42 in the retinal samples of cholesterol-fed rabbits compared to normal rabbits. D. BACE-1 and E. IDE levels are increased in retinas from cholesterol-fed rabbits. *p < 0.05, ***p < 0.001 vs control.