Figure 2

Retinas of cholesterol-fed animal exhibit an increase in oxidative stress. A. The cholesterol-enriched diet significantly increased ROS levels in the retinas as demonstrated by DCFH-DA. B, C. Fluorimetric detection of H₂O₂ and peroxidase activity by Amplex Red Assay showed an increase in H₂O₂ levels in cholesterol-fed rabbit retinas and decreased peroxidase activity. D. HO-1 levels were increased in the retinas of cholesterol-fed rabbits as shown by Western blotting. *p < 0.05;**p < 0.01; ***p < 0.001 vs control.