Cholesterol-enriched diet disturbed cholesterol homeostasis in the retina. A. Cholesterol-fed rabbit retinal sections showed an increase in the expression of CYP27A1 (green). DAPI (blue) is a nuclear counter stain. ONL, INL, GCL are outer nuclear layer, inner nuclear layer and ganglion cell layers respectively. B. Western blotting further confirmed an increase in the expression of CYP27A1. C. Western blotting results showed that ABCA-1 levels were increased in the retinas of cholesterol-fed rabbits for facilitating excess cholesterol efflux. *p < 0.05, **p < 0.01 vs control. Bar = 20 μm.