Figure 2

Retina histological findings (H&E staining, x 200). An endothelin-1 dosage of 0.1 μg/day was delivered for two (II), four (III), and eight weeks (IV), and balanced salt solution was delivered for eight weeks (Ib) to the perineural region of the anterior optic nerve. Compared to Group Ib, the number of cells in the retinal ganglion cell layer (long arrow), the inner nuclear layers (short arrow), and the outer nuclear layer (green arrowhead) were remarkably decreased in Groups II, III, and IV. Vacuoles without a nucleus (empty arrowhead) were found in endothelin-1-treated groups. In addition, thinning of the inner and outer nuclear layers and the inner and outer plexiform layers was noted. The outer limiting membrane, composed of the zonula adherens, exhibited loosening and widening (yellow arrowhead).