Figure 1

Preparation of an enucleated porcine eye and side-view imaging technique. A, The eye was frozen and transected at an angle of about 45 degrees to the equatorial plane with a utility knife. B and C, The bisected eye is fixed to a glass slide with a ring of superglue covered with wheat flour. The eye was left at room temperature for 3 minutes and a tight seal developed between the bisected eye and glass slide. D, Photograph of the equipment used for side-view cinematography.