Detection of autoantibodies against heat shock proteins and collapsin response mediator proteins in autoimmune retinopathy

Table 1 Identification of human retinal proteins found in spots cut out of a Coomassie-stained 2D gel by mass spectrometry

Spot number	Major protein(s)	Accession	Spectral counts
1	Heat shock cognate 71 kDa protein	HSP7C_HUMAN	89
2	Heat shock cognate 71 kDa protein	HSP7C_HUMAN	59
2	Heat shock 70 kDa protein 1A/1B	HSP71_HUMAN	30
3	Stress-70 protein, mitochondrial	GRP75_HUMAN	29
3	Heat shock cognate 71 kDa protein	HSP7C_HUMAN	22
4	Heat shock cognate 71 kDa protein	HSP7C_HUMAN	14
4	60 kDa heat shock protein, mitochondrial	CH60_HUMAN	8
5	Alpha-internexin	AINX_HUMAN	10
	Vimentin	VIME_HUMAN	7
	Dihydropyrimidinase-related protein 2	DPYL2_HUMAN	6
6	60 kDa heat shock protein, mitochondrial	CH60_HUMAN	40
7	60 kDa heat shock protein, mitochondrial	NUCB1_HUMAN	18
	Alpha-internexin	AINX_HUMAN	11
	Pyruvate kinase isozymes M1/M2	KPYM_HUMAN	8
8	Nucleobindin-1	NUCB1_HUMAN	11
	Alpha-internexin	AINX_HUMAN	10
	Alpha-1-antitrypsin	A1AT_HUMAN	8
	Rab GDP dissociation inhibitor alpha	GDIA_HUMAN	7
	Protein kinase C and casein kinase	PACN1_HUMAN	6
	substrate in neurons protein 1
9	Rab GDP dissociation inhibitor alpha	GDIA_HUMAN	8
	Nucleobindin-1	NUCB1_HUMAN	7
	Pyruvate kinase isozymes M1/M2	KPYM_HUMAN	6
	Alpha-1-antitrypsin	A1AT_HUMAN	5
10	Succinyl-CoA:3-ketoacid coenzyme A transferase 1	SCOT1_HUMAN	17
11	Stress-70 protein, mitochondrial	GRP75_HUMAN	81
12	Dihydropyrimidinase-related protein 2	DPYL2_HUMAN	57
13	Dihydropyrimidinase-related protein 3	DPYL3_HUMAN	28
14	Dihydropyrimidinase-related protein 3	DPYL3_HUMAN	21
15	Dihydropyrimidinase-related protein 3	DPYL3_HUMAN	20
16	Dihydropyrimidinase-related protein 3	DPYL3_HUMAN	14
17	60 kDa heat shock protein, mitochondrial	CH60_HUMAN	60

Spots were cut out of a Coomassie-stained 2D gel of human retinal proteins in a molecular weight range from approximately 60–70 kDa and pI of 5.0-6.5 and subjected to in-gel digestion with trypsin. See Figure 3 for spot numbers.

ISSN: 1471-2415