Figure 2

Laser Microdissection of OS epithelial regions. The histological OS epithelial sections used for LMD were stained with toluidine blue dye. All the images in the composite are at 20x magnification. Composite shows tissue sections in different stages of LMD. Images A, D and J represents preLMD corneal, limbal and conjunctival epithelium with horizontal graphic lines outline dividing the epithelial width into segments. Images B, E, H and K represent post LMD sections of corneal, limbal, LEC and conjunctival epithelium. The graphic outline around the segments is joined to facilitate cutting with laser and the cut segments were catapulted in the collection tubes (images C, F, I and L). Image G represent preLMD section of LEC, with RoboLPC function the LEC was dissected and catapulted in the collection tube (Image H). In image H LEC tissue was sectioned into half to facilitate catapultion in the collection tube. The dot seen at the end of the midsection cut marks the point where the laser pulse catapults the tissue in the collection tube by RoboLPC function (Image I).