Figure 4

Composite image of nanodrop spectrophotometry of amplified and labelled probes of spotted oligonucleotide microarrays. Images A and B are nanodrop spectrophotometry of second round of amplification and labelled probe of a microarray sample (C0404 LEC) respectively. These graphs were used to determine the quality of the amplification and labelling of the sample for further processing. Image 4A of the amplified RNA demonstrates RNA concentration and 260/280 ratio which shows absence of protein contamination. The overall graph curve indicates good quality of amplified RNA. Image 4B of the labelled probe gives the concentration of the sample with frequency of incorporation of the dye in the sample.