Table 1 Details of samples processed for spotted microarray showing LMD area, corresponding concentration, of unamplified, amplified and labelled RNA samples with FOI values

SC-CO398 Cornea

233,571

52.87

368.5

423.2

32

SC-CO399 Cornea

223,254

92.88

160.7

309

32

SC-CO418 Cornea

200,597

40.77

270.7

319.9

30.25

SC-CO398 Limbus

221,436

47.41

301.6

394.9

40

SC-CO399 Limbus

246,046

49.24

359.7

176.5

32.6

SC-CO404 Limbus

310,000

66.68

717.5

120.3

34.7

SC-CO418 Limbus

212,236

47.16

248.8

220

38

SC-CO398 LEC

86,251

35.95

232.3

127.2

35.2

SC-CO399 LEC

192,689

32.03

429.6

79.3

42

SC-CO404 LEC

279,404

80.59

581.2

249.2

41.3

SC-CO418 LEC

122,123

48.54

154.5

134.2

22

SC-CO399 Conj

214,212

24.54

274.1

151.1

41

SC-CO404 Conj

213,909

48.39

289.5

366.1

30

SC-CO418 Conj

151,862

62.3

330.4

254.6

41.27

SC-CO398 LEC Stroma

302,869

53.86

392

73.6

38

SC-CO399 LEC Stroma

392,887

46.03

542.8

109.4

46

SC-CO404 LEC Stroma

283,421

36.67

624.8

293.5

40.9

SC-CO418 LEC Stroma

320,055

26.31

324.1

551.9

33

Ch2: source name Corneal + Conj epithelium

RNA conc μg/μl

2nd Round amplified RNA ng/μl

Labelled extract ng/μl

FOI of Cy3 dye

SP-C0479 SP1

2.22 +8.98

912.9

641.9

31.6

SP-C0479 SP2

912.9

496.7

40