Figure 1
From: Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice
Assessment of retinal vascularization. (a, b) Angiographic analysis by fluorescein-dextran. (c, d) Histological demonstration of retinal neovascularization. (a, c) Normal mice. (e) Quantification of retinal neovascularization. (b, d) Oxygen-induced retinopathy (OIR) mice. (a) Normal mice, retinal vessels form a polygonal reticular pattern. (b) OIR mice, neovascular tufts appear as hyperfluorescence at the junction between the perfused and nonperfused areas (indicated by red arrows). The nonperfused areas are marked with an asterisk. (c) Normal mice, no neovascular cell nuclei extend beyond the internal limiting membrane. (d) OIR mice, many neovascular cell nuclei protrude into the vitreous cavity (indicated by arrows). GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Bar = 50 μm.