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Figure 1 | BMC Ophthalmology

Figure 1

From: Lipofuscin accumulation and autophagy in glaucomatous human lamina cribrosa cells

Figure 1

The ultra-structural appearance of LC cells and their accumulation of lipofuscin. A: The TEM images shown demonstrate the ultra-structural appearance of normal and glaucomatous LC cells at low (7450X) and high (22300X) power magnifications. Note the abundance, particularly in LC cells from the glaucoma donors, of predominantly peri -nuclear, lysosome-like, membrane bound organelles containing an amorphous, electron dense material suggestive of lipofuscin. Two such structures have been indicated in the lowest frame by an arrow. B: Quantification of lipofuscin-like lysosomes in each of 10 randomly selected peri-nuclear fields at magnifications of 7450X and/or 22300X were recorded for normal and glaucomatous LC cells. These structures were more numerous in LC cells from glaucoma donors compared to those from normal donors (4.1 × 10,000 per h.p.f. ± 1.9 vs. 2.0 × 10,000 per h.p.f. ± 1.3, p = 0.002, n = 3). C: The area of each of the lipofuscin like lysosomes identified for the construction of Figure 1B as described above was calculated twice using ImageJ software and a mean value for each recorded. Lipofuscin occupied a larger cell area in glaucomatous LC samples when compared to normal LC samples (46.8 mm2 ± 2.9 vs. 23.9 mm2 ± 0.3, p = 0.07, n = 3).

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