Figure 3

Expression and localization of WRN proteins in human retinas. WRN protein signals (green) were detected in the inner nuclear layer (INL; yellow arrows) (A) and the outer nuclear layer (ONL; white arrow) (B, C). The WRN protein signals co-existed with vimentin signals (red; yellow arrows) (A) but not with GFAP signals (red; C). These results indicate that the WRN proteins are expressed in the cytosol of Müller cells in human retinas. The upper picture of Panel D shows the rhodopsin signals (red) coexisted with WRN signals (green) in the ONL (D). The lower picture of Panel D shows the cone arrestin signals (red) coexisted with WRN signals (green) in the ONL (D). Panel E shows expression and localization of WRN proteins in epiretinal membrane of another patient with proliferative diabetic retinopathy. WRN protein signals (green) were identified in the nucleus (E). Blue signals showed DAPI-stained nuclei. Blue signals showed DAPI-stained nuclei. GCL; ganglion cell layer, INL; inner nuclear layer, ONL; outer nuclear layer, VM; vimentin, GFAP; glial fibrillary acidic protein. Bars = 20 μm.