Method used to evaluate the corneal neovascularization (CNV). A: The Corneas were divided into thirds from epithelial surface to endothelium. B: Sample of a cornea coloured with hematoxylin and eosin (h&e). Blue lines show the virtual division in thirds of the corneas. The vessels identified in each field (black arrows) counted as positive. C: Schematic cornea showing 40X fields from the periphery to the center. D: Sample of a cornea stained with h&e, black circles marked the 40X fields used to measure CNV extension.