Skip to main content
Figure 3 | BMC Ophthalmology

Figure 3

From: Oxidative stress causes ERK phosphorylation and cell death in cultured retinal pigment epithelium: Prevention of cell death by AG126 and 15-deoxy-delta 12, 14-PGJ2

Figure 3

H2O2 induced lipid peroxidation in RPE. ARPE-19 cells were treated with 1.5 mM H2O2 for 4 hours then processed for immunofluorescent staining using antibodies against 4-HNE. Fig. 3A: untreated cells. Scale bar: 50 μm. Fig. 3B: H2O2 treated cells. Note the overall enhanced staining in nucleus and cytoplasm. Fig. 3C: ARPE-19 cells were treated with various concentrations (0.5, 1, 1.5, 2 mM) of H2O2 for 24 hours then processed for Western blot analysis using anti-4-HNE antibodies. These polyclonal antibodies recognize cysteine-, histidine- and lysine-4-HNE Michael adducts. This is a representative result from 6 similar experiments.

Back to article page