Figure 5
Experimental biofouling studies with F. solani (238-06) and F. oxysporum (158-06). Fungal cells (1 × 105) were inoculated into 1.0 ml of MoistureLoc solution, and 100-μl aliquots were plated on yeast extract-peptone-dextrose agar at intervals (a-h). Fungal colonies were recovered from MoistureLoc® solutions sampled at 15, 30 and 60 min intervals (b-d) F. solani; (f-h) F. oxysporum. All solutions were sterile after the manufacturer recommended length of treatment, 4 hr (data not shown). The total numbers of colonies recovered are ten-fold of the dilution shown in these illustrations; the data is summarized in figure 6. Similar results were seen when 3-months old and freshly opened MoistureLoc solutions were compared. The experiments were repeated at least three times; a representative experiment is shown.