Table 1 The sequences of primers and their relative binding sites on 18S rRNA gene of representative species
From: Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis
Primer name | Sequence 5'............3' | Location (position of base pair) | ||
|---|---|---|---|---|
| Â | Â | Aspergillus niger (GenBank accession no. D63697) | Fusarium solani (GenBank accession no. E17084) | Candida sp. (GenBank accession no. EF120586) |
PFPRIM-F3 | GACTCAACACGGGGAAACT | 1149–1167 | 1222–1240 | 1134–1152 |
PFPRIM-R4 | ATTCCTCGTTGAAGAGCA | 1522–1539 | 1605–1622 | 1509–1526 |