Figure 4

phenotype of CD133 ⁺ retinal cells following 24 hour culture. The Figure demonstrates histograms of gated CD133 events showing relative expression of stem/progenitor cell markers that change when cells are cultured with either FGF/N2 or FGF-LIF overnight. Expression of Notch-1 (A), CD135 (B), CRALBP (C), Nestin (D), Pax-6 (E), DBX (F) changed on CD133⁺ retinal cells following culture overnight with FGF/N2 (dotted line) or FGF-LIF (dashed line). Grey filled peaks are isotype mAb background fluorescence for cells cultured in FGF/N2 as MFI did not significantly change when cells were cultured in either culture condition.