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Figure 5 | BMC Ophthalmology

Figure 5

From: CD133+adult human retinal cells remain undifferentiated in Leukaemia Inhibitory Factor (LIF)

Figure 5

FGF2/N2 optimises generation of neurospheres from CD133 + retinal cells. Primary, CD133+ and CD133 depleted cells were cultured for 1 week, post-sort, with FGF2, FGF2/N2, FGF2-LIF and FGF2/N2 -LIF. Non parametric statistical analysis for neurosphere generation at 1 week (A) Neurosphere generation was enhanced when cells were cultured in FGF2/N2: At 1 week † Primary:CD133+:depleted P < 0.02, * Primary:CD133+ P < 0.02 (n = 14). At 2 weeks: † Primary:CD133+:depleted P < 0.01, *Primary:CD133+ P < 0.03 (n = 10). Neurosphere generation/100000 was significantly reduced in the presence of LIF (§ ¤ P < 0.05).(B) Supernatants from CD133+ cultures were removed following 2 days, 1 week and 2 weeks and analysed for LIF production (pg/ml). LIF (pg/ml) increased over 2 weeks. However it was noted that CD133+ retinal cells contained less LIF (pg/ml) over 2 weeks in culture which corresponded to increased neurosphere generation.

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