Skip to main content
Figure 10 | BMC Ophthalmology

Figure 10

From: αA-crystallin R49Cneomutation influences the architecture of lens fiber cell membranes and causes posterior and nuclear cataracts in mice

Figure 10

Cryoimmuno electron microscopic analysis of wild type and R49C neo /R49C neo knock-in lenses. Lenses expressing either wild type or heterozygous R49Cneo/R49Cneo αA-crystallin were labeled with an antibody to αA-crystallin and analyzed by cryoimmuno electron microscopy. (A) Wild type lenses showed linear fiber cell membranes. Immunogold labeling (black dots) showed that αA-crystallin was restricted to the cytoplasm. (B) Heterozygous WT/R49Cneo αA-crystallin lenses had several gold particles on the fiber cell membranes. Furthermore, the linearity of lens fiber cell membranes was lost, and significant gaps between cells were apparent.

Back to article page