Fungal structural changes before and after cryotherapy by SEM (3500×). After treatment, the cells of Candida albicans expanded or shrunk, and the cell wall and the cell membrane was fractured. The hyphae of Fusarium solaniand Aspergillus fumigates were diminished or fractured. Cells of the pathogenic fungal crushed, and the cell wall and the cell membrane was fractured. Arrows indicate the detailed changes due to cryotherapy.