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Fig. 5 | BMC Ophthalmology

Fig. 5

From: Protective effect of 14-3-3 antibodies on stressed neuroretinal cells via the mitochondrial apoptosis pathway

Fig. 5

Expression of 14-3-3 sigma in RGC-5 revealed by indirect immunfluorescence. RGC-5 cells were fixed, permeabilised, blocked and incubated with rabbit polyclonal anti 14-3-3 antibodies. Subsequently the cells were incubated with Goat polyclonal secondary antibody to rabbit IgG-H&L conjugated with FITC. The cells were visualized with a fluorescence microscope. The pictures show that 14-3-3 was expressed in all cells and it seems to be distributed in the cytoplasm

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