Fig. 2

The surgical procedure for Ologen™ collagen matrix (OCM) graft and conjunctival autograft surgery. a,b The size of the scleral defect was measured and its margin was marked with a 3-mm diameter biopsy punch to determine the boundary of conjunctivectomy, c The margin of scleral thinning area was trimmed by Vannas scissors and the OCM was cut with a circular-shaped biopsy punch of the same size (3-mm diameter). The OCM was trimmed and fitted to cover the scleral defect. d The OCM was sutured with a recipient scleral wall using six stitches of 10–0 nylon interrupted sutures. The color of the OCM changed from white to red due to blood accumulation. e A 4-mm diameter, circular, free CAU was harvested from the superonasal bulbar conjunctiva with a punch biopsy 1 mm larger in diameter than that of the piece of OCM. f The CAU was carefully positioned over the previously sutured OCM bed. g The CAU was anchored to the scleral wall and the healthy conjunctival margin through the OCM bed with 11 stitches of interrupted sutures of the 10–0 nylon. h Immediate final postoperative appearance