Fig. 1

Premature senescence in mice endothelium of corneal dysfunctional allografts after PKP a Clinical evaluation of mouse corneal graft in syngeneic group (after 4.5 months post grafting); b Evaluation of endothelium of corneal graft in syngeneic group (n = 4); c Representative results of SA-β-Gal staining on corneal endothelium in syngeneic group; d Representative results of 8-hydroxydeoxyguanosine (8-OHdG) staining on corneal endothelium in syngenic group; e Clinical evaluation of mouse corneal graft in allogenic group (after 4.5 months post grafting); f Evaluation of endothelium of corneal graft in allogenic group; g Representative results of SA-β-Gal staining on corneal endotheliumin in allogenic group; h Representative results of 8-OHdG staining on corneal endothelium in allogenic group. Rejection of corneal grafts was observed in allogenic group as opacification of the cornea and new vessel in growth, compared with corneal grafts in syngeneic group e. In syngeneic group, the original hexagonal structure was maintained b compared with corneal grafts in allogenic group f. The endothelial cell borders of corneal grafts in allogeneic group were not clear, and polykaryocytes were observed in corneal endothelium of allogeneic group f. Compared with corneal grafts in allogeneic group g, SA-β-Gal positive cells were not observed on corneal endotheliumin in syngeneic group c. This revealed that dysfunctional corneal allografts exhibited characteristics of premature endothelial senescence. Compared with corneal grafts in syngenic group d, the strength and numbers of positive cells of 8-OHdG staining were less than that in allogenic group h