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Fig. 2 | BMC Ophthalmology

Fig. 2

From: Oxidative stress and premature senescence in corneal endothelium following penetrating keratoplasty in an animal model

Fig. 2

Up-regulated expression of p16INK4A, p21Cip1 and p53 proteins in mice endothelium of dysfunctional corneal allografts Expression of cell senescence related proteins, p16INK4A , p21Cip1/CDKN1A and p53 in mice endothelium of dysfunctional corneal allografts. Changes in protein expression as determined by Western blot. a data from the gels; b normalization to GAPDH. For each sample, the relative abundance of the protein of interest is determined by calculating the ratio of the intensity of the signal for the protein of interest to that of the normalization control GAPDH. Band densities determined by ImageJ software and compared with syngenic group. Expression of p16INK4A, p21Cip1/CDKN1A and p53 were higher in the corneal endothelium of allogenic group than in the syngenic group (t-test, P < 0.05, n = 3). Significant differences between the corneal endothelium tissue in syngenic and allogenic groups are indicated by an asterisk (*P < 0.05)

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