Fig. 8

ASK1/p38 signaling is required for the response to ROS in cultured HCECs in vitro. a The level of ROS was measured and compared with or without SB203580 treatment of HCECs under H₂O₂ induced oxidative stress conditions in vitro. Bar = 100 μm. The HCECs with no SB203580 treatment was used as control. Three more additional experiments achieved equivalent results. b SA-beta-Gal staining was performed and compared with or without SB203580 treatment of HCECs under H₂O₂ induced oxidative stress conditions in vitro. Bar = 25 μm. The HCECs with no SB203580 treatment was used as control. Three more additional experiments achieved equivalent results. c The protein level of p16, p21 and p53 was measured by Western blot and compared with or without SB203580 treatment of HCECs in vitro. Left panel: shows the representative data from the gels; right panel: the results normalized to GAPDH. GAPDH served as the loading control. The HCECs with no SB203580 treatment was used as control. Three more additional experiments achieved equivalent results. Data are means ± SD (n = 3). All means marked with *(P < 0.05) are significantly different from the control