Fig. 3

Immunodot blot analysis of inflammatory cytokine profiles in culture medium of human corneal fibroblasts (HCFs) with or without angiogenin (ANG) treatment. a Treatment of HCFs with tumor necrosis factor-alpha (TNF-α, 20 ng/ml) resulted in amplification of five inflammatory cytokines and chemokines including growth regulated oncogene (GRO), TNF-α, interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1. Treatment of HCFs with lipopolysaccharide (LPS, 1 μg/ml) resulted in amplification of five inflammatory cytokines and chemokines including GRO, TNF-α, IL-6, IL-8, and MCP-1. Treatment of HCFs with ANG (2 μg/ml) resulted in reduction in expression of these inflammatory cytokines and chemokines. b Cytokine Antibody Array Map. Pos: positive control; Neg: negative control; ENA: epithelial neutrophil-activating protein; GCSF: granulocyte colony-stimulation factor; GM-CSF: granulocyte macrophage CSF; IFN-γ: interferon-gamma; MCSF: macrophage CSF; MDC: monocyte chemotactic protein; MIG: monokine induced by IFN-γ; MIP: macrophage inflammatory protein; RANTES: Regulated on Activation, Normal T Cell Expressed and Secreted; SCF: stem cell factor; SDF-1: stromal cell derived factor-1; TARC: thymus and activation-regulated chemokine; TGF-β1: transforming growth factor-beta1; EGF: epidermal growth factor; IGF-1: insulin-like growth factor; VEGF: vascular endothelial growth factor; PDGF: platelet-derived growth factor. c Relative density of inflammatory cytokines and chemokines. The values presented in the bar graph are the mean ± standard error from triplicate experiments. One-way ANOVA followed by Bonferroni’s post hoc analysis, *p < 0.05 versus TNF-α without ANG; #p < 0.05 versus LPS without ANG