Fig. 1

Transcription factor binding sites Prediction. The sequence around the CpG site, which displays the most significant differences in methylation between nuclear ARC cases and controls, is analyzed for transcription factor binding prediction via TESS website. a Several transcription factor-binding sites are predicted in the sequence. The most frequent predicted transcription factor is Sp1. b Transcription factor binding in seleted sequence. We shorten the sequence and select one, which mainly binds to Sp1 for further study. c Transcription factor binding in mutated sequence. If the “cg” site mutate to “ag” site, no transcription factor binding is found