Fig. 3

Histopathology and immunohistochemistry of the surgically excised corneal button. a Histopathology of the central cornea. There were many small vesicles (10 to 100 μm in diameter) the corneal stroma. The vesicles were much more numerous in the deep stroma than in the upper stroma (hematoxylin and eosin staining; magnification × 40). b Histopathology of the central cornea. Accumulations in the vesicles were alcian-blue positive (alcian-blue staining; magnification × 40). c Collagen VI immunostaining of the central cornea detected collagen VI in some of the vesicles (magnification × 40)