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Fig. 2 | BMC Ophthalmology

Fig. 2

From: Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia

Fig. 2

Immunofluorescence microscopy analysis of Keratin degradation and Fibronectin enhancement by CoCl2 stimulated on SRA01/04 cells. Human SRA01/04 cell monolayers were cultured on coverslips and 48 h after CoCl2 treatment, coverslips were analyzed by immunofluorescence microscopy. Red represents Fibronectin and green represents Keratin. Images of SRA01/04 cells: 12 h cultured without CoCl2 treatment (a-c), 48 h without CoCl2 treatment (d-f); 48 h treated with CoCl2 (h-j). treated only with second antibodies (k-m). n Quantification of the percentage of Keratin-positive SRA01/04 cells, relative to DAPI-stained nuclei. o Quantification of the percentage of Fibronectin-positive SRA01/04 cells, relative to DAPI-stained nuclei

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