Fig. 3

Fluorescence-activated cell sorting (FCAS) separates endothelial cells from Apln-CreERT:mT/mG and Cdh5-CreERT:mT/mG mice and higher apelin expression in GFP positive endothelial cells of Apln-CreERT:mT/mG mice. a The scatter dot plot (left) and histogram (right) of FACS analysis before and after FACS sorting of green meGFP endothelial cells. b ECs before and after FACS sorting under the fluorescent microscope. c The FACS sorted ECs are VE-Cadherin positive. d Apelin mRNA expression in mGFP positive endothelial cells from Apln-CreERT:mT/mG is significantly higher than the cells from Cdh5-CreERT:mT/mG mice. e Apelin expression in eGFP positive endothelial cells is significantly higher than tdTomato endothelial cells of Apln-CreERT: mT/mG mice. Data quantification was mean ± S.E.M (n = 6). All data were analyzed using Student’s t-test unless otherwise noted. n.s., not significant; *, P < 0.05. Scale bars: b, 100 μm; c, 50 μm