Fig. 1

Schematic illustration showing the reculturing methods used to evaluate antibiofilm effect of nitric oxide. 1) Soft contact lens was incubated with bacteria for 1–7 days. 2) To analyse the biofilm attached to each contact lens, only the contact lens was harvested and transferred to fresh culture media for additional 24-h culture. 3, 4 & 5 ) After 24 h of re-culture, the bacterial burden dispersed in the culture media was analysed by optical density measurement and colony forming unit counting