Fig. 3
From: The Antibiofilm efficacy of nitric oxide on soft contact lenses
Human corneal epithelial cell viability assay. Human corneal epithelial cell viability was measured using CCK-8 kit after 3 (a), 6 (b), 24 (c) and 48 h (d) of incubation with various concentrations (0.1–1000 μM) of NaNO2. The graphs showed a mild increase in cell viability with NaNO2 addition at 6, 24 and 48 h. No detrimental effect of NaNO2 on cell viability was observed at the tested concentrations. Triplicates of each treatment group were used in each independent experiment. Values are the mean ± standard error of means from three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001)