Fig. 4
From: The Antibiofilm efficacy of nitric oxide on soft contact lenses
Nitric oxide’s effect on contact lens associated biofilm analyzed by optical density analysis. Optical density (OD) of samples was converted to relative density (%) (the OD of the sample divided by OD of the negative control) and was shown in bar graphs. a–c OD comparison in samples with Staphylococcus aureus. The OD was lower than the negative control at all concentrations of NaNO2 at days 1 and 3. The effect of NaNO2 on biofilm formation was dose dependent. At day 7, low concentrations (0.1 and 1 μM) of NaNO2 decreased the biofilm but failed to reach statistical significance. Higher concentrations (10–1000 μM) of NaNO2 significantly decreased the biofilm formation even at day 7. d–f OD comparison in samples with Pseudomonas aeruginosa. The OD was lower than the negative control at all concentrations of NaNO2 at days 1 and 3. The effect of NaNO2 on biofilm formation was dose dependent. At day 7, low concentrations (0.1 μM) of NaNO2 decreased the biofilm but failed to reach statistical significance. Higher concentrations (1–1000 μM) of NaNO2 significantly decreased the biofilm formation even at day 7. Statistical significance was determined using one-way analysis of variance (ANOVA) followed by the Tukey HSD test. *Statistically significantly higher than control group, **Statistically significantly higher than 0.1 μM group.***Statistically significantly higher than 1 μM group,****Statistically significantly higher than 10 μM group,*****Statistically significantly higher than 100 μM group