Fig. 5
From: The Antibiofilm efficacy of nitric oxide on soft contact lenses
Nitric oxide’s effect on contact lens-associated biofilm analyzed by colony forming unit counting analysis. a and b Representative pictures of agar plates showing the colonies after 24 h of reculturing. Colony forming unit (CFU) count decreased with increasing concentrations of NaNO2 in both S. aureus and P. aeruginosa. The number of scattered colonies decreased with increasing concentrations of NaNO2. c–h The CFU count of the samples was converted to the ratio (%) (the CFU count of the sample divided by CFU count of the negative control) and was shown in bar graph. The CFU count of both S. aureus and P. aeruginosa shows dos-dependent decrease with increasing concentration of NaNO2. The suppressing effect of NaNO2 decreased at day 7 in biofilm formed by P. aeruginosa. Statistical significance was determined using one-way analysis of variance (ANOVA) followed by the Tukey HSD test. *Statistically significantly higher than control group, **Statistically significantly higher than 0.1 μM group.***Statistically significantly higher than 1 μM group,****Statistically significantly higher than 10 μM group,*****Statistically significantly higher than 100 μM group