Fig. 2From: Phospholipase Cγ2 is critical for Ca2+ flux and cytokine production in anti-fungal innate immunity of human corneal epithelial cellsEngagement of Dectin-1 activates PLCγ2 in HCECs. a HCECs were treated with 5 × 106/ml A. fumigates hyphae for various times as indicated their activation status determined by Western Blot. b HCECs were preincubated for 30 min with Dectin-1 inhibitor Laminarin, followed by the treatment of hyphae for 15 min. The activation of PLCγ was assayed by Western Blot. Data shown are representative of more than three independent sets of experiments. *p < 0.05, **p < 0.001Back to article page