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Table 2 Primer sequences and condition used for PCR and Sanger sequencing of UBIAD1 gene

From: Schnyder corneal dystrophy and associated phenotypes caused by novel and recurrent mutations in the UBIAD1 gene

Target

Forward primer

Reverse Primer

Size (bp)

Enzyme

Annealing Temp

exon 1

CCGTCCTTCCTCCTTCCC

AAGCCACCTTTGACATCCCT

700

GoTaqGreen

65 °C

exon 2

CCACCTGCACAGTCTAAGGA

CTGCCAAATCACATTCCTTCCT

689

GoTaqGreen

60 °C