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Table 1 Primers used for amplification and sequence analysis of human PAX6

From: Identification of a novel PAX6 mutation in a Chinese family with aniridia

Exon

Forward Sequence 5′-3′

Reverse Sequence 5′-3′

EXON04

AGATCGCCCCAAGAGGTTG

ATCGAGAAGAGCCAAGCAAAC

EXON05

GGTGGTCCTGTTGTCCTTTAT

GGGGTCCATAATTAGCATCGT

EXON06–07

AGCTCTCTACAGTAAGTTCTCA

CCCAGGTACAAAGGAGACAAA

EXON08

TCCGCCCAATTCTCTATCCAA

TACACAACCCTCACATTCCCA

EXON09

GGTGAGGCTGTCGGGATATAA

TCTTTGTACTGAAGATGTGGCA

EXON10–11

TAACTTGGTTCTGGTGGGAAA

CGGAGCAAACAGGTTTAAAGA

EXON11–12

TGCTAACCTGTCCCACCTG

GAAAAGCTCTCAAGGGTGCAG

EXON12–13

AGGCTTGATACATAGGCAGCT

GGACAAGGAAAGCAAGGAGTT

EXON14

TGTATTCCATGTCTGTTTCTCA

GGTACAATACAGGACACAATTG

  1. Note: All primers were amplified using a touchdown protocol beginning at 64 °C, decreasing
  2. by 0.5 °C each cycle, until finishing at a final annealing temperature of 57°°C
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BMC Ophthalmology

ISSN: 1471-2415

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