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Fig. 1 | BMC Ophthalmology

Fig. 1

From: All-trans retinoic acid stimulates the secretion of TGF-β2 via the phospholipase C but not the adenylyl cyclase signaling pathway in retinal pigment epithelium cells

Fig. 1

ATRA stimulated the expression of TGF-β2 mRNA in D407 cells. D407 cells were treated with 10 μM ATRA for 2, 4, 8, 16, 24, and 48 h, and the expression of TGF-β2 protein was detected by western blot analysis. a The electrophoretogram of TGF-β2 protein in the 10 μM ATRA-treated and control groups for 2, 4, 8, 16, 24, and 48 h. It was found that 10 μM ATRA stimulated the expression of TGF-β2 protein in a time-dependent manner. b After treatment with ATRA for 2 h, the level of TGF-β2 protein in D407 cells was increased significantly compared with that of the control group (p < 0.001) and peaked at 16 h. However, there were no statistically significant differences in the level of TGF-β2 protein at 16 h, 24 h and 48 h (p > 0.05; n = 6 per treatment)

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BMC Ophthalmology

ISSN: 1471-2415

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