Skip to main content
Fig. 7 | BMC Ophthalmology

Fig. 7

From: Effects of concentration of amyloid β (Aβ) on viability of cultured retinal pigment epithelial cells

Fig. 7

Relationship between RAGE and Aβ in the expression of VEGF and PEDF. Si RAGE-inserted ARPE-19 cells were incubated with Aβ 1–40 for 24 h, and the expression of the mRNA of VEGF-A and PEDF were measured by real-time RT-PCR using β-actin as an endogenous control. The control in each group was defined as 1 and show the number of relative comparisons in the experimental group. After 48 h of incubated with Aβ 1–40, the living cell number was measured by WST-8 assay. Knockdown of RAGE attenuated the increase and decrease of VEGF (a) and PEDF (b) expression caused by Aβ. In addition, Si-RAGE attenuated the increase and decrease of viable RPE cell number induced by Aβ addition (c). Data are the means ± SEMs for each group (n = 4)

Back to article page