Fig. 1
From: Autophagy protects against retinal cell death in mouse model of cytomegalovirus retinitis
Effect of rapamycin treatment on mTOR, autophagy and apoptosis during MCMV retinitis. BALB/c Mice were immunosuppressed and injected via the supraciliary route with MCMV in a volume of 2 μl, and then injected with rapamcyin (rapa, 1.25 mg/kg/day) intraperitoneally every day. The inoculated eyes were collected at day 7 p.i. and prepared for fluorescent staining for MCMV early antigen (EA), TUNEL and DAPI (a). b The number of TUNEL-positive cells in rapamycin treated retinas was compared to that of non-treated retinas during MCMV retinitis. c Immunoblotting probed with antibody against LC3B, caspase 3, and phosphorylated mTOR (c), and virus titer (d) were performed respectively. The images in (a) are 400×. MCMV+rapa: MCMV infected and rapamycin treated mice