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Table 1 Sequences of primers used for genotyping mice

From: Autophagy protects against retinal cell death in mouse model of cytomegalovirus retinitis

Gene Primer sequence Predicted band size (in bp)
Atg5 WT Forward: 5′-GAATATGAAGGCACACCCCTGAAATG-3′; Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′ 350
Atg5 flox Forward: 5′-ACAACGTCGAGCACAGCTGCGCAAGG-3′; Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′ 700
Atg5 deleted Forward: 5′-CAGGGAATGGTGTCTCCCAC-3′; Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′ 320
Cre Internal CT Forward: 5′-CTAGGCCACAGAATTGAAAGATCT-3′ Reverse: 5′-GTAGGTGGAAATTCTAGCATCATCC-3′ 324
Cre Forward: 5′-GCGGTCTGGCAGTAAAAACTATC-3′ Reverse: 5′-GTGAAACAGCATTGCTGTCACTT-3′ 100