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Table 1 Sequences of primers used for genotyping mice

From: Autophagy protects against retinal cell death in mouse model of cytomegalovirus retinitis

Gene Primer sequence Predicted band size (in bp)
Atg5 WT Forward: 5′-GAATATGAAGGCACACCCCTGAAATG-3′;
Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′
350
Atg5 flox Forward: 5′-ACAACGTCGAGCACAGCTGCGCAAGG-3′;
Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′
700
Atg5 deleted Forward: 5′-CAGGGAATGGTGTCTCCCAC-3′;
Reverse: 5′-GTACTGCATAATGGTTTAACTCTTGC-3′
320
Cre Internal CT Forward: 5′-CTAGGCCACAGAATTGAAAGATCT-3′
Reverse: 5′-GTAGGTGGAAATTCTAGCATCATCC-3′
324
Cre Forward: 5′-GCGGTCTGGCAGTAAAAACTATC-3′
Reverse: 5′-GTGAAACAGCATTGCTGTCACTT-3′
100