Fig. 1

Preliminary Experiment. Gross photographs of porcine tarsal plate after 30-min treatment with standard 0.1% riboflavin in isotonic 20% dextran (“standard”), 0.1% riboflavin in isotonic 20% dextran with 0.05% benzalkonium chloride (BAK) (“isotonic + BAK”), 0.1% riboflavin in hypotonic 20% dextran (“hypotonic”), and 0.1% riboflavin in hypotonic 20% dextran with 0.05% BAK (“hypotonic + BAK”). Conjunctiva was dissected off in one group (“conjunctiva OFF”), and left intact for an additional group of specimens (“conjunctiva ON”). Greater tissue stiffness was observed grossly in treatment specimens as compared to controls, and this effect seemed to be greater in “conjunctiva OFF” treatment specimens, but photographs lacked standardization