Fig. 2

Gross Photographs and “Flop”. (a) Gross photographs of porcine tarsal plate specimens using a standardized metal clamp mounted in a fixed location to the camera and a registration image. Images were auto-aligned via position in Adobe Photoshop using the registration marks and then exposure corrected for contrast. Pre-crosslinking treatment and post-crosslinking treatment photographs are shown for representative control (C2), treatment specimen with least “delta flop” (T1), and two treatment specimens with greatest “delta flop” (T4 and T9). (b) Composite image of all photographed porcine tarsal plate specimens (10 control, 10 60-min treatment) using a standardized metal clamp mounted in a fixed location to the camera and a registration image, demonstrating a change in “flop” for the post-treatment group. All tested control and treatment specimen images were auto-aligned via position in Adobe Photoshop using the registration marks and then exposure corrected for contrast. The overlay image was created using the Layer Difference Blending Mode. *P < 0.0001