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Fig. 2 | BMC Ophthalmology

Fig. 2

From: Histopathologic study of extraocular muscles in thyroid-associated ophthalmopathy coexisting with ocular myasthenia gravis: a case report

Fig. 2

Histopathologic changes in TAO + OMG, TAO and control extraocular muscle samples. a Hematoxylin-eosin stain (× 400) showed diffuse inflammation and myofiber enlargement in the TAO + OMG and TAO samples. The cross-sectional area of each myofiber was calculated based on the largest diameter (solid line) perpendicular to the longitudinal axis (dashed line) measured in 3 randomly chosen fields of each slide. The myofiber size was larger in the TAO + OMG (289.9 ± 142.5 μm2) and TAO (544.1 ± 160.6 μm2) samples than the control samples (157.0 ± 47.7 μm2). The variation of myofiber size was smaller in the TAO (± 29.5% around average) and control (± 30.4% around average) samples than the TAO + OMG samples (± 49.2% around average). The bars represent the 95% confidential interval. The box represents the mean and standard deviation. b Alcian blue stain (× 400) demonstrated accumulation of glycosaminoglycan (blue) in the TAO + OMG and TAO samples. The proportion of glycosaminoglycan was quantified in 3 randomly chosen fields of each slide based on the fraction of positive-stained pixels in the total pixels of the interstitial space. The glycosaminoglycan-occupied area was larger in the TAO + OMG samples (48.8 ± 12.2%) than the TAO (28.4 ± 3.6%) and control (3.3 ± 0.8%) samples. c Masson stain (× 400) revealed increased collagen fibers (blue) in the TAO samples but not in the TAO + OMG samples. The proportion of collagen fibers in the interstitial space was quantified in 3 randomly chosen fields of each slide based on pixel counting. The collagen-occupied area was larger in the TAO samples (60.5 ± 6.4%) than in the TAO + OMG (36.1 ± 4.3%) and control (33.9 ± 2.7%) samples

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