Fig. 3
Other histopathologic changes in TAO + OMG, TAO and control extraocular muscle samples. a The immunostaining of αSMA (× 400), a potential marker of fibrosis, revealed stronger reactivity (brown) in the TAO samples than in the TAO + OMG and control samples. b The periodic acid Schiff stain (× 400) demonstrated glycogen (red) in the muscle fibers, showing endomysial aggregation of glycogen in the TAO samples and segmental deposition of glycogen in the TAO + OMG samples. c Muscle types were distinguished based on the ATPase stain (× 200) of frozen sections, with weak intensity for type I fibers and strong intensity for type II fibers. Same fiber types tended to group together in the TAO + OMG samples, resulting in predominantly type I fibers in some fascicles (dotted line) and type II fibers in others (dashed line). d Central nuclei (white arrowheads) were frequently observed in the TAO + OMG samples on hematoxylin-eosin stain (× 400) but not in the TAO or control samples. e The Gomori’s trichrome stain (× 400) of frozen sections revealed mitochondrial distribution (red) in the TAO + OMG samples, showing both a normal pattern of linear distribution (white arrowheads) and an abnormal pattern of focal aggregation (black arrowheads)