Fig. 2From: MiR-221/SIRT1/Nrf2 signal axis regulates high glucose induced apoptosis in human retinal microvascular endothelial cellsMiR-221 aggravates HG-induced apoptosis in hRMEC cells. a: qRT-PCR analysis of miR-221 level in hRMEC transfected with miR-221 mimics or inhibitor as indicated. (** indicates P < 0.01; *** indicates P < 0.001). b: MTT analysis of viability of HG included hRMEC transfected with miR-221 mimics or inhibitor as indicated. (* indicates P < 0.05; ** indicates P < 0.01). c: Apoptosis analysis of HG included hRMEC transfected with miR-221 mimics or inhibitor as indicated by FACS. d: Statistical analysis of FACS results in c. (* indicates P < 0.05; ** indicates P < 0.01). e: WB detection of cleaved-caspase 3, Bcl-2 and Bax expression in hRMEC transfected with miR-221 mimics or inhibitor as indicated. Full-length blots are presented in Supplementary Figure 2E. Statistical analysis of relative expression was conveyed and displayed in columns. (* indicates P < 0.05; ** indicates P < 0.01)Back to article page